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Increased Gene Expression in Mammalian Cell Lines using pAdVAntage™ DNA as a
Co-Transfectant
Deb Groskreutz and Elaine Schenborn
Promega Corporation
Promega's pAdVAntage™ Vector carries the Adenovirus VAI RNA gene, a gene required
for the efficient translation of cellular and viral genes in adensovirus-infected cells.
When pAdVAntage™ DNA is co-transfected with luciferase constructs, an approximately
27-fold increase in luciferase expression is observed in 293 and HeLa cell lines over
transfections performed with the construct DNA alone.
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