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Mutational Analysis Using in vivo Suppression of Amber Stop Codons:
The INTERCHANGE™ in vivo Amber Suppression Mutagenesis System
Scott A. Lesley, Joe Ziegelbauer, Mark A. Maffitt
Promega Corporation
Amber suppressor tRNAs have been isolated which allow insertion of any one of 12
amino acids at an amber (TAG) stop codon. Using a set of strains containing these various
suppressor tRNAs, it is possible to determine the effect of substituting each of these 12
amino acids at a particular site by making a single amber mutation.
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