Staining Nucleic Acids with Silver: An Alternative to Radioisotopic and Fluorescent
Labeling
Gustavo Caetano-Anollés* and Peter M. Gresshoff
Plant Molecular Genetics
Institute of Agriculture and Center for Legume Research
The University of Tennessee
Knoxville, TN 37901-1071
*To whom correspondence should be addressed.
Nucleic acids separated on polyester-backed polyacrylamide gels can be detected
consistently at the picogram level using a very simple acidic silver stain. The procedure
is fast, has relatively few steps and reagents, and produces the least number of staining
artifacts.
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