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Developing Meaningful Assays for the Eukaryotic Transcription Reagents TFIIB and
Anti-TFIIB
Elaine Schenborn, Mark Knuth, Stephanie Vavra and Virgina Goiffon
Promega Corporation
The general transcription factor TFIIB is an integral component of the RNA
polymerase II initiation complex. Promega now offers human recombinant TFIIB and an
Anti-TFIIB monoclonal antibody. In this article, we describe the functional assays we
developed for this factor and antibody. We also compare our preparations of recombinant
TFIIB with those from another supplier and demonstrate the higher degree of purity and
activity of the Promega preparations.
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