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Use of the CytoTox 96™ Assay in Routine Biocompatibility Testing In Vitro
Matthew J. Allen and Neil Rushton
University of Cambridge Orthopaedic Research Unit
Addenbrooke's Hospital
Cambridge, United Kingdom
The complete evaluation of potential biomaterials should include testing for overt
toxicity, long-term foreign body responses and mutagenicity. The release of lactate
dehydrogenase from cultured cells exposed to a biomaterial provides a sensitive and
accurate marker for cellular toxicity. Materials which cause overt toxicity in this
screening procedure should be dismissed as candidates for use in vivo. The CytoTox
96(TM) Assay is a fast, accurate and cost-effective test for LDH release that is as
applicable to routine biocompatibility testing as it is to immunological studies of
cellular cytotoxicity.
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