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Luminometry for In Vivo and In Vitro Reporting of Firefly Luciferase
Bruce A. Sherf and Keith V. Wood
Promega Corporation
The Turner Designs Model 20 Luminometer is a sensitive, versatile and economical
luminometer designed specifically for use with firefly luciferase. Our results show that
the Model 20, a direct current luminometer, is equivalent in sensitivity to a quality
luminometer that employs photon-counting to quantify light emission. In addition, the
Model 20 has a unique sample compartment that is adaptable to different sample tube sizes
and optical filters, and allows for direct measurements of luminescence in living cells
and tissues.
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