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Large-scale Gel Purification of DNA Fragments using Wizard™
PCR Preps Resin
Chuck York, David Birschbach and Shauna Navarro
Promega Corporation
Many procedures in molecular biology require the purification of specific DNA fragments
away from other DNA fragments, including vector DNA, nonspecific amplification products or
other contaminating fragments generated in a restriction digestion. Moreover, a number of
applications call for large amounts of specific DNA fragments. Gel purification of DNA is
a convenient means of isolating high-quality DNA fragments in large amounts. While many
investigators avoid large-scale gel purification, this technique can be much more rapid
and cost-effective than multiple small-scale preparations.
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