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A practical guide to DNA methylation
Tom Schoenfeld
Promega Corporation
The pattern and extent of DNA methylation can significantly affect the success of
restriction digestions or bacterial transformations. Prokaryotic DNA may be methylated by
host restriction/modification systems, while eukaryotic DNA often is methylated in CG or
CNG di- or trinucleotides. This article presents background information on the effects of
methylation on common cloning applications as well as troubleshooting tips for situations
where DNA methylation interferes with the handling of DNA.
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