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Stabilized TMB Substrate for Horseradish Peroxidase vs. 4-chloro-1-naphthol: A
comparison on Western blots
Gregg Larson
Promega Corporation
TMB Stabilized Substrate for Horseradish Peroxidase was compared to
4-chloro-1-naphthol on Western blots. TMB Stabilized Substrate gave at least 3-fold
greater immunodetection sensitivity. In addition, the color produced by this new substrate
developed faster and was less susceptible to fading.
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