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RNase ONE™: Advantages for nuclease protection assays
Gary Brewer
Dept. of Microbiology and Immunology
Bowman Gray School of Medicine of
Wake Forest University
Medical Center Blvd.
Winston-Salem, NC 27157-1064
Elizabeth Murray and Matt Staeben
Promega Corporation
RNase ONE offers several advantages over other enzymes used in nuclease protection
assays. It cleaves 3´ to all bases, enabling more accurate mapping than with mixtures of
RNase A and T1. Because of the high purity of cloned RNase ONE, less optimization is
required to obtain a reproducible signal level. RNase ONE also is inhibited by 0.1% SDS,
permitting ethanol precipitation of the protected RNA fragments without prior
phenol:chloroform extractions.
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