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A novel method for non-radioactive assays of specific protein kinases
Douglas White and John Shultz
Promega Corporation
We report the development of a novel, non-radioactive protein kinase assay which
employs dye-conjugated peptide substrates for specific protein kinases. The peptides have
been designed so that phosphorylation causes a change from a +1 to a -1 net charge,
allowing rapid separation and identification of the reaction products. Unlike assays
utilizing 32P incorporation, this assay is not subject to interference from
phosphorylation of other substrates present in crude samples.
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