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Transcriptional activation assays of recombinant transcription factors
Elaine Schenborn, Ray Bandziulis, Rita Joshi, Jude Stevens and Ken Lewis
Promega Corporation
Christopher R. Bartley and Peggy Farnham
University of Wisconsin-Madison
Transcriptional activation assays provide key functional tests of the activity of
recombinant transcription factors. Nuclear extracts lacking the specific factor to be
tested are obtained by antibody depletion, by heat treatment, or from naturally depleted
sources. In this report, we describe transcriptional activation assays which have been
developed for quality assurance testing of SP1 and TFIID (TATA-binding protein).
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