|
Download
article
191kb
pdf? |
|
Recombinant Clone Screening Using the GoTaq® Hot Start Green
Master Mix
Sarah Wheeler
Promega Corporation
We describe using the new GoTaq® Hot Start Green Master Mix to
screen recombinant clones from single colonies on a plate and from
plasmid minipreps. The master mix formulation contains direct-to-gel
loading components, which provide convenience and allow fast PCR setup.
With the GoTaq® Hot Start Polymerase technology, reactions
can be assembled and stored at room temperature for up to 24 hours prior
to cycling.
|
