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A Guide to Optimizing Protein Synthesis in the S30 T7
High-Yield Protein Expression System
Kate Qin Zhao and Don Creswell
Promega Corporation
Optimal protein yield with the S30 T7 High-Yield Protein Expression System
relies on a number of variables including: having a suitable expression
vector, using a high-quality DNA template, adding the right amount of
template to the reaction, and finding the best reaction time and
temperature for your protein. Genetic manipulations of the
protein-coding sequence, such as codon usage, truncation/domain and
fusion tags can also be explored.
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