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HaloTag® Technology: Convenient, Simple and Reliable
Labeling from Single Wells to High-Content Screens
Hélène A. Benink
Promega Corporation
The advent of fluorescent protein labeling technology has revolutionized
cell biology by providing the means to study precise localization and
dynamics of proteins within living cells. As this technology has
evolved, there have been great advances both in the number of spectrally
distinct fluorescent labels available and in the microscopes used to
detect them. In fact, elegant studies that include distinct fluorescent
entities used together in single cells now have become commonplace in
the literature. However, the conventional fluorescent reporting
technologies themselves exhibit one fundamental drawback to the
scientist: each of the fluorescently labeled proteins requires
individual cloning of the gene of interest fused to the fluorescent tag
sequence. In other words, researchers must make a separate construct for
each “color” of fluorescent tag they wish to put on a protein of
interest. The HaloTag® labeling technology eliminates this
time-consuming issue for biologists.
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