|
Download
article
115kb
pdf? |
|
The S30 T7 High-Yield Protein Expression System
Kate Qin Zhao, Don Creswell and Michael Slater
Promega Corporation
The S30 T7 High-Yield Protein Expression System is an E. coli
extract-based cell-free protein synthesis system that transcribes and
translates DNA sequences cloned in vectors containing a T7 promoter or a
strong E. coli promoter. Protein yield can reach hundreds of
micrograms per milliliter of reaction (up to 500 µg/ml) within 1 hour at
37 ºC. The reaction volume can be scaled down to as little as 5 µl for
high-throughput applications.
|
