|
Download
article
186kb
pdf? |
|
Transfection-Quality Plasmid DNA in as Little as Ten Minutes
Using the PureYield™ Plasmid Miniprep System
Don Smith and Eric Vincent
Promega Corporation
Scientists require straightforward methods for plasmid purification as the
first step in many laboratory protocols. Here we introduce the new
PureYield™ Plasmid Miniprep System, which allows researchers to isolate
high-quality plasmid DNA suitable for a broad range of downstream
applications, including transfection, in vitro expression, sequencing
and cloning. The system allows direct purification of plasmid from as
much as 0.6 ml of bacterial culture without cell harvesting or from up
to 3 ml of culture when the cells are pelleted. The entire procedure can
be completed in as little as 10 minutes.
|
