PureYield™ Plasmid Miniprep
I really like these new PureYield™ Plasmid minipreps!
However, I have to be very careful when working with PureYield™ Plasmid preps. Not only are they fast - 10 minute minipreps! (I have to avoid the sun), but PureYield™ purified DNA is really pure. My luciferase assays produce so much light, I have to wear sunscreen!!
Promega pGL4.13 plasmid was prepared from 1.5ml overnight cultures using a competing plasmid miniprep system or the PureYield™ Plasmid Miniprep System. Five different commonly used mammalian cell lines were transfected with the plasmid, and transfection efficiency was assessed by measuring the luciferase activity of the cell lines using the ONE-Glo™ Luciferase Assay System. (N = 6).
The Lysis/Neutralization indicator dye allows me to be sure that I am mixing my samples appropriately
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The blue to yellow color change after neutralization ensures that mixing is complete.
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