The Ultimate in Reporter Gene Assays

ONE-Glo™ Luciferase Assay Reagent provides unequaled, robust performance while eliminating many of the handling inconveniences that may be experienced when using reporter assays, especially in an HTS environment.

ONE-Glo uses novel a firefly luciferase substrate,
5'-fluoroluciferin

Reconstituted ONE-Glo Reagent can be used much longer than other Bright-type reagents

ONE-Glo reagent protects the luciferase reaction from inhibition by 10 µM resveratrol, an known firefly luciferase inhibitor

ONE-Glo Reagent is more tolerant of phenol red than luciferin-based reagents

Extended bright light output allows high sensitivity assays to be run in batch or continuous operations

    

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• Simplify Your Assay Optimization: The robust performance, reduced odor, improved storage, and larger available sizes make assay optimization even easier and more efficient.
• Store at Room Temperature or at 4°C: The extended stability of the ONE-Glo™ Reagent at room temperature and 4°C makes it more convenient for everyday use.
• Improve Assay Precision: Because the ONE-Glo™ Reagent is less sensitive to mixing and dispensing conditions, reproducibility is enhanced. The assay is ideal for use in high-density (384- and 1536-well) microplates.
• Get a Brighter, Longer Signal: Optimized for batch and continuous-process handling, the extended bright light output of the ONE-Glo™ Assay allows high sensitivity, especially when extended incubation is required prior to reading results.
• Reduce Unwanted Effects from Sample Components: The novel chemistry used in the ONE-Glo™ Assay is less sensitive to culture media, phenol red, and luciferase inhibitors than other luciferase assays.