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Neural Notes

Abstract

Live/Dead Assay: In situ labeling of apoptotic neurons with CaspACE™ FITC-VAD-FMK Marker

Mary L. Michaelis and Yingxue Chen (Corresponding author Email: mlm@ukans.edu) Dept. of Pharmacology/Toxicology, University of Kansas, Lawrence, KS 66045 USA

In this short report, embryonic (day 18) rat brain cortical neurons were plated and induced to undergo apoptosis (by staurosporine addition). The cell-permeable FITC conjugate of the caspase inhibitor, VAD-FMK, CaspACE™ FITC-VAD-FMK In Situ Marker, was used to detect apoptotic cells. Immunostaining results are shown.

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