Death Check™ Assay Systems

The Death Check™ I (Cat.#G7370), II (Cat.# G7380) and III (Cat.# G7390) Assay Systems are bundled combinations of two systems, outlined below, for measuring different aspects of apoptosis. Each Death Check™ System contains a brief Technical Bulletin explaining the contents of the specific systems as well as the full Technical Bulletins for each individual component system.

Anti-pT¹⁸³ MAPK pAb, Rabbit

Anti-pT¹⁸³ MAPK pAb, Rabbit (Cat.# V8081), is raised against a peptide corresponding to ERK1 and ERK2 enzymes monophosphorylated on threonine residue 183. This affinity-purified antibody is recommended for use at a 1:4,000 dilution in Western analysis with cellular extracts. This antibody is also suitable for immunostaining at a 1:250 dilution.

PC12 Cell Extracts, Western Controls

PC12 Cell Extracts, Western Controls, were designed for use with the Anti-ACTIVE^® MAPK and JNK pAbs. Each contains both treated and untreated cell extracts for use in Western blotting analysis. The NGF (nerve growth factor)-treated cell extract (Cat.# V8110) is ideal as a positive control for detecting activated ERK1 and ERK2. The sorbitol-treated cell extract (Cat.# V8100) is ideal as a positive control for detecting activated JNK1 and JNK2.

Anti-PARP p85 Fragment pAb

Anti-PARP p85 Fragment pAb (Cat.# G7341) is directed against the 85kDa caspase-cleaved fragment (p85) of human poly(ADP-ribose) polymerase (PARP). The specificity for the cleaved form of PARP makes this antibody a novel marker for early detection of apoptosis. The antibody is designed for use as an immunocytochemical/immunohistochemical marker for apoptosis in cells and tissues.

Nde II

New restriction enzyme, Nde II (Cat.# R7291)
Source: Neisseria dentrificans Recognition Site: 5^'…^vGATC…3'
Isoschizomers: Mbo I, Dpn I (neoschizomer G^meA^vTC) and Sau3A I
Nde II is sensitive to dam methylation (same as Mbo I)

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