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Parallel Analysis of BDNF and NT-3 in Rat Brain
by David J. Earnest, Rajesh Miranda and Farida Sohrabji (Corresponding
author: dearnest@tamu.edu) Texas A&M University, Health Science Center Department of
Human Anatomy and Medical Neurobiology College of Medicine College Station, TX 77843-1114
Reverse transcription-PCR is a powerful approach to detect
rare transcripts in a specific tissue. However, one problem with focusing exclusively on
message abundance is that mRNA expression for a given gene may not be indicative of levels
of its protein product. In this report, we demonstrate the concurrent analysis of mRNA and
cognate protein for BDNF and NT-3 in mammalian rat brain.
PDF article (69kb)
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