Tfx^TM-20 Reagent and Gene Delivery into Mouse CNS

Andrew I. Brooks¹ and Howard J. Federoff^1,2 Corresponding author: A.I. Brooks, 9130@frontiernet.net Departments of Microbiology and Immunology¹, and Department of Neurology², University of Rochester School of Medicine and Dentistry, Rochester, NY 14642 USA

Gene transfer efficiency of two viral vectors, two synthetic cationic lipid reagents and naked DNA were evaluated in mice injected unilaterally in two brain regions. Animals received 1µl over a 10-minute period of either HSVlacZ, AdCMVlacZ, Tfx^TM Reagent*/HSVlacZ plasmid complex or naked DNA (HSVlacZ plasmid). After four days, animals from each group were perfused and tissue was prepared for histochemical detection of beta-galactosidase expression. beta-galactosidase positive cells confined to the injection site were observed in the HSV, adenovirus and Tfx^TM-20 Reagent groups only at the injection site in animals injected using a micropump. To quantify expression, tissue punches harvested from the injection sites and other brain regions were analyzed using a chemiluminescent reporter assay.

Correction: Panel E of Figure 2 in this article is incorrect. The correct panel appears in Volume IV, Issue 2, p. 23. We apologize for this error.

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