CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay

The CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay (Cat.#  G3580, G3581, G3582) provides an increased level of convenience by combining the novel tetrazolium compound MTS*, [3-(4, 5-dimethylthiazol-2-yl)-5-(3 carboxymethoxyphenyl)-2-( 4-sulfophenyl)-2H-tetrazolium, inner salt], and the electron coupling reagent phenazine ethosulfate (PES). The CellTiter 96^® AQ_ueous One Solution Reagent is added directly to cells; no pre-mixing of components is needed. Because of its stability and the fact that the MTS reduction product does not require solubilization, the CellTiter 96^® AQ_ueous Cell Proliferation Assay requires fewer steps than other cell viability assays that contain tetrazolium compounds (e.g., MTT and INT). All of the CellTiter 96^® AQ_ueous Cell Proliferation Assays are non-radioactive, colorimetric assays for determining the number of viable cells in proliferation and cytotoxicity assays. MTS (Owen’s reagent) is bioreduced by cells into a colored formazan product that is soluble in tissue culture medium. The convenient “One Solution” format represents an improvement over the current CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay where phenazine methosulfate (PMS) is used as the electron coupling reagent, and solutions of PMS and MTS are supplied separately.

CellTiter 96^® AQ_ueous MTS Reagent Powder

CellTiter 96^® AQ_ueous MTS Reagent Powder (Cat.# G1111, G1112) is now available in 250mg for those who perform intermediate scale proliferation assays. MTS is the tetrazolium dye found in all CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assays. Colorimetric measurement of cellular proliferation is monitored by the conversion of MTS into a soluble formazan product. PMS (phenazine methosulfate) is not supplied and must be purchased separately. For more information on the use of this product, please request the CellTiter 96^® AQ_ueous MTS Reagent Powder Technical Bulletin #TB169.

E_max^® ImmunoAssay Systems Family

Over the past year, Promega has introduced five systems featuring the robust methodology of the enzyme immunoassay (EIA). These systems feature specific mono- and polyclonal antibodies for the detection of soluble glial cell-line derived neurotrophic factor (GDNF; Cat.# G7620, G7621), nerve growth factor (NGF; Cat.# G7630, G7631) (introduced in this issue), neurotrophin-3 (NT-3; Cat.# G7640, G7641) and transforming growth factors (TGFs) beta1 (Cat.# G7590, G7591) and beta2 (Cat.# G7600).

NGF E_max^® ImmunoAssay Systems*

Promega recently developed the NGF E_max ImmunoAssay System (Cat.# G7630, G7631)  for the sensitive detection of NGF in a variety of neuroscience research applications.  This system allows accurate quantitation of NGF in serum or tissue homogenates and can detect NGF in many mammalian species. These systems contain sufficient reagents for 160 or 400 determinations of experimental samples, plus human NGF standards in an antibody sandwich format.

GDNF E_max^® ImmunoAssay Systems*

The GDNF E_max^® ImmunoAssay System (Cat.# G7620, G7621) is now available in a convenient 2 x 96 well plate size for those who perform fewer assays, or for those desiring to test the system for their specific application in small pilot trials. This system allows accurate quantitation of GDNF in the range of 16-1,000pg/ml. The specificity of the GDNF E_max^TM ImmunoAssay Systems were tested with recombinant human NGF and TGFalpha, and natural human, TGFbeta1, which showed less than 2% cross-reactivity.

Nerve Growth Factor, 2.5S, Murine

Promega now offers its popular mNGF, 2.5S, in a convenient 10µg size (Cat.# G5142) for those requiring smaller quantities of this important growth factor. Promega’s 2.5S mNGF, supplied lyophilized, exhibits an ED₅₀ value below 2ng/ml using a PC12 neurite outgrowth assay.

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