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Neural Notes

Abstract

ELISA in situ: A Sensitive Tool for Detecting Release of Endogenous BDNF

by Agnieszka Balkowiec, M.D., Ph.D. and David M. Katz, Ph.D., Department of Neurosciences, Case Western Reserve University School of Medicine

This article describes measurement of endogenous BDNF release from neurons using Promega's BDNF Emax® ImmunoAssay Systems (Cat.# G7611, G7610). The ELISA in situ method incorporates the coating antibody into the culture system and dramatically increases the detection of BDNF in both control and stimulated neuron cultures.

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