Information webinar

In the genomics era, the information flow goes from obtaining sequence information to evaluating gene expression and determining the subsequent protein function. The biological research focus is now moving toward a systematic characterization of genes and their encoded proteins from the perspective of their expression and function in the cellular context (proteomics).

Multiple approaches may be used to determine the many levels of protein function, including predictive in-silico bioinformatics, expression profiling, extract-based, cell-based and whole animal assays.

In-vitro protein expression systems offer significant time-saving over other approaches traditionally used to study aspects of gene product function. It is mostly based on cell-free translation of RNA and on coupled eukaryotic systems (TNT) which allow rapid protein expression directly from DNA, included PCR-generated fragments. Although the process of coupled transcription/translation is complex, the use of rapid in-vitro expression systems for many different applications is simple, convenient and versatile.

The use of cell-free systems for the in-vitro expression of proteins is a rapidly growing and constantly evolving area, with applications in basic research, molecular diagnostics and high-throughput screening that we will introduce.

This webinar was given on September 15, 2008: 11:00 - 12:00.
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