Promega @cademy:
Other interesting educational tools
In
order to complete the information related to Promega @cademy
activities, here are some links to interesting
educational tools, especially animations and videos.
Some of these were developed by Promega, others were
found on the internet and considered as interesting so
that we wanted to share them with you.
Bioluminescent assays
Introduction to Bioluminescence Assays (view
animation)
Introduction to Reporter Gene Assays (view
animation)
Apoptosis
Apoptosis Overview (view
animation).
Intrinsic Triggering of Apoptosis (view
animation).
RNA interference
Introduction to
RNA interference (view
animation).
psiSTRIKE™ Vectors and RNA Interference (view
animation).
Cell-based assays
MultiTox-Fluor
Multiplex Cytotoxicity Assay (view
animation).
Culture Preparation and Plating for Cell-Based Assays (view
video).
PCR
Introduction to
PCR (view
animation).
Introduction to the Plexor™ Systems (view
animation).
Genotyping with the Plexor™ qPCR System (view
animation).
Protein expression and analysis
HaloCHIP™ System (view
animation)
HaloTag® Interchangeable Labeling Technology (view
animation).
Single Step (KRX) Competent Cells (view
animation).
The Flexi® Cloning System
(view
animation).
TNT® SP6 High-Yield Protein Expression System
(view
animation).
Protein-Protein Interactions and the TNT® Systems (view
animation).
Protein-DNA Interactions and the TNT® Systems
(view
animation)
Co-immunoprecipitation to Study Protein-Protein
Interactions Using the TNT® Systems (view
animation).
Signal transduction
Inner Cell
Summary:
The Inner Life of a Cell is an animation created in
NewTek LightWave 3D and Adobe After Effects for Harvard
biology students which illustrates unseen molecular
mechanisms and the ones they trigger, specifically how
white blood cells sense and respond to their
surroundings and external stimuli.
View video:
http://www.youtube.com/watch?v=Et5mGi6yEeM
In-vivo imaging
Summary
The discovery of human embryonic stem cells (hESCs) has
dramatically increased the tools available to medical
scientists interested in regenerative medicine. However,
direct injection of hESCs, and cells differentiated from
hESCs, into living organisms has thus far been hampered
by significant cell death, teratoma formation, and host
immune rejection. Understanding the in vivo hESC
behavior after transplantation requires novel imaging
techniques to longitudinally monitor hESC localization,
proliferation, and viability. Molecular imaging has
given investigators a high-throughput, inexpensive, and
sensitive means for tracking in vivo cell proliferation
over days, weeks, and even months. This advancement has
significantly increased the understanding of the spatio-temporal
kinetics of hESC engraftment, proliferation, and
teratoma-formation in living subjects. A major advance
in molecular imaging has been the extension of
noninvasive reporter gene assays from molecular and
cellular biology into in vivo multi-modality imaging
platforms. These reporter genes, under control of
engineered promoters and enhancers that take advantage
of the host cell s transcriptional machinery, are
introduced into cells using a variety of vector and
non-vector methods. Once in the cell, reporter genes can
be transcribed either constitutively or only under
specific biological or cellular conditions, depending on
the type of promoter used. Transcription and translation
of reporter genes into bioactive proteins is then
detected with sensitive, noninvasive instrumentation
(e.g. CCD cameras) using signal-generating probes such
as D-luciferin. To avoid the need for excitatory light
to track stem cells in vivo as is required for
fluorescence imaging, bioluminescence reporter gene
imaging systems require only an exogenously administered
probe to induce light emission. Firefly luciferase,
derived from the firefly Photinus pyralis, encodes an
enzyme that catalyzes D-luciferin to the optically
active metabolite, oxyluciferin. Optical activity can
then be monitored with an external CCD camera. Stably
transduced cells that carry the reporter construct
within their chromosomal DNA will pass the reporter
construct DNA to daughter cells, allowing for
longitudinal monitoring of hESC survival and
proliferation in vivo. Furthermore, because expression
of the reporter gene product is required for signal
generation, only viable parent and daughter cells will
create bioluminescence signal; apoptotic or dead cells
will not. In this video, the specific materials and
methods needed for tracking stem cell proliferation and
teratoma formation with bioluminescence imaging will be
described.
View the video on:
http://www.jove.com/index/details.stp?id=740
Introduction to the MAPK Pathway
The Mitogen-Activated Protein Kinase (MAPK) signaling
pathways modulate many cellular events including: the
cell cycle, embryonic development, movement, apoptosis,
and neuronal differentiation (view
animation).
The PI3K Pathway
This animation demonstrates some events associated with
the phosphoinositol 3-kinase pathway (view
animation).
Miscellaneous
Promega animations, videos and tools:
-
Animations and Video's
-
Tools
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