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    Case Study:
    Problem: A scientist really liked the Promega Dual-Luciferase Reporter 1000 Assay System (DLR) for screening but found the packaging cumbersome.

    Solution: Promega provided the kit components only, without the kit box itself. This made it easier to use the system and saved important freezer space.


    Case Study:
    Problem: A scientist needed to move his DLR assay from 96-well to 384-well, but without a 384 injector instrumentation.

    Solution: Promega reformulated Dual-Glo to give the scientist a brighter signal with a shorter half-life so that he could work in the 384-well format.



    Case Study:
    Problem: Biotech firm looking to change suppliers because of poor service. They came to Promega even though the second strand buffer they needed was not a catalog product.

    Solution: Promega created the second strand buffer product. Its performance not only paralleled the old product, it exceeded performance of what the biotech firm had been using.




    The Caspase-Glo 3/7 Assay provides superior sensitivity and linearity over a broad range of cell numbers.





    Superior sensitivity of the Apo-ONE Assay compared to standard fluorescent caspase assays. Results are obtained in as little as 1 hour.





    The Caspase-Glo 8 Assay (A) or Caspase-Glo 9 (B) Assay provides superior sensitivity and linearity over a broad range of cell numbers.





    General protocol for Promega’s luminescent and fluorescent homogeneous caspase assays.

    If you initially measure… Then you may measure…  
         
    Cell Viability Caspase Activity
    or General Apoptosis
     
    CellTiter-Blue™ Cell Viability Assay (fluorescent) Apo-ONE® Homogeneous Caspase-3/7 Assay (fluorescent)
         
    Initiator Caspase Activity Executioner
    Caspase-3/7 Activity
     
    Caspase-Glo™ 8 Assay
    (bioluminescent)
    Apo-ONE® Homogeneous Caspase-3/7 Assay (fluorescent)
    Caspase-Glo™ 9 Assay
    (bioluminescent)
    Apo-ONE® Homogeneous Caspase-3/7 Assay (fluorescent)
         
    Cytotoxicity or Necrosis Caspase  Activity or General Apoptosis  
    CytoTox-One Homogeneous Membrane Integrity Assay
    (fluorescent, modified protocol)
    Caspase-Glo™ 3/7 Assay (bioluminescent)
         
    Reporter Gene Activity Caspase  Activity
     
     
    EnduRen™ Live Cell Substrate for Renilla luciferase activity (bioluminescent) Apo-ONE® Homogeneous Caspase-3/7 Assay  
      Caspase-Glo™ 3/7 Assay (bioluminescent)
      Caspase-Glo™ 8 Assay (bioluminescent)
      Caspase-Glo™ 9 Assay (bioluminescent)
    Contact Technical Services for details    





    The APO-ONE Assay can produce excellent Z′-factor values. Z′-factor value = 0.83 for this assay.





    The Caspase-Glo 3/7 Assay can produce excellent Z′-factor values. Z′-factor value = 0.92 for this assay.





    The Caspase-Glo 8 Assay can produce excellent Z′-factor values.
    Z′-factor Value = 0.957 for this assay.





    The Caspase-Glo 9 Assay can produce excellent Z′-factor values.
    Z′-factor Value = 0.903 for this assay.



    Request Literature about Promega's Caspase Assays

         
    Apo-ONE® Homogeneous Caspase-3/7 Assay Caspase-Glo™ 3/7 Assay Caspase-Glo™ 8-9 Assays

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