Technical Resources
Animations and Videos
Bioluminescent Assays
Introduction to Bioluminescence Assays
This animation shows how we exploit the chemistry of the bioluminescent reaction to create a suite of robust and sensitive biological assays. Genetic Reporter Assays, ATP Assays, and Luciferin Substrate Assays are presented."(view animation)
Introduction to Reporter Gene Assays
This animation introduces the concept of reporter gene assays, using the Dual-Luciferase® Assay as a model.(view animation)
The GloMax™ Integrated Luminescence System
Learn how the GloMax™ Luminometers are integrated with Promega assays and technical support. Take a tour of each instrument, see the assay procedure, and explore data analysis and system performance using either the GloMax™ 20/20 or the GloMax™ 96 Luminometer. (view animation)
Apoptosis
Apoptosis Overview
This animation provides a brief overview of programmed cell death. (view animation)
Intrinsic Triggering of Apoptosis
This animation provides a brief explanation of a possible intrinsic apoptotic pathway. In this case, damage to nuclear DNA triggers a cascade of events that triggers apoptosis. (view animation)
Cell-Based Assays
MultiTox-Fluor Multiplex Cytotoxicity Assay
This animation explains the advantages of a multiplexed cell-based assay for measuring cytotoxicity. (view animation)
Culture Preparation and Plating for Cell-Based Assays
This video demonstrates how to prepare and plate a culture for use in a cell-based assay. (view video)
PCR
Introduction to PCR
This animation provides a step-by-step introduction to this important molecular biology technique. (view animation)
Introduction to the Plexor™ Systems
The Plexor™ Systems are a new way to perform quantitative PCR. Understand the basic concepts behind the Plexor™ technology, then see how the Plexor™ Systems are used to quantify a single target DNA sequence or multiple target sequences. (view animation)
Genotyping with the Plexor™ qPCR System
See how the Plexor™ qPCR System can be used in genotyping assays to distinguish between two alternative bases at a specific site in a known DNA sequence. (view animation)
Protein Expression and Analysis
HaloCHIP™ System
Learn more about the HaloCHIP™ protocol; a ChIP assay with no antibody required. (view animation)
HaloTag® Interchangeable Labeling Technology
This animation introduces you to a specially designed fusion protein that can be covalently tagged with an interchangeable set of ligands for use in multiple applications, including cellular imaging, protein imobilization, and capture of binding proteins. (view animation)
Single Step (KRX) Competent Cells
Explore the features of these competent cells designed for efficient transformation of protein expression vectors and tightly controlled protein expression. (view animation)
The Flexi® Cloning System
This animation demonstrates the use of the Flexi® Vector System for directional cloning of protein-coding sequences and the transfer of these sequences between a variety of Flexi® Vectors without the need to resequence. (view animation)
TNT® SP6 High-Yield Protein Expression System
See the advantages of the TNT® High Yield System over conventional wheat germ and E. coli systems. (view animation)
Protein-Protein Interactions and the TNT® Systems
See how to use the MagneGST™ and TNT® Systems to detect protein-protein interactions between the products of cloned genes. (view animation)
Protein-DNA Interactions and the TNT® Systems
See how to use the TNT® Systems to study protein-DNA interactions in applications such as gel shift assays. (view animation)
Co-immunoprecipitation to Study Protein-Protein Interactions Using the TNT® Systems
See how to use the TNT® Systems to study protein-protein interactions using co-immunoprecipitation. (view animation)
Purification
RNA Interference
Introduction to RNA interference
This animation introduces the basic concept of RNA interference and provides an overview of DNA-directed RNA interference. (view animation)
psiSTRIKE™ Vectors and RNA Interference
See how the siSTRIKE™ U6 Hairpin Cloning Systems use linearized plasmids to easily clone hairpin target sequences and express siRNA target sequences in human cells. (view animation)
Signal Transduction
Introduction to the MAPK Pathway
The Mitogen-Activated Protein Kinase (MAPK) signaling pathways modulate many cellular events including: the cell cycle, embryonic development, movement, apoptosis, and neuronal differentiation. (view animation)
The PI3K Pathway
This animation demonstrates some events associated with the phosphoinositol 3-kinase pathway. (view animation)