Technical Resources
Animations and Videos
Bioluminescent Assays
Introduction to Bioluminescence Assays
This animation shows how we exploit the chemistry of the bioluminescent reaction to create a suite of robust and sensitive biological assays. Genetic Reporter Assays, ATP Assays, and Luciferin Substrate Assays are presented." (view animation)
Introduction to Reporter Gene Assays
This animation introduces the concept of reporter gene assays, using the Dual-Luciferase® Assay as a model. (view animation)
Studying Gene Regulation
This animation illustrates the use of reporter gene assays to study transcriptional and post-transcriptional regulation of the Transferrin Receptor gene. (view animation)
The GloMax™ Integrated Luminescence System
Learn how the GloMax™ Luminometers are integrated with Promega assays and technical support. Take a tour of each instrument, see the assay procedure, and explore data analysis and system performance using either the GloMax™ 20/20 or the GloMax™ 96 Luminometer. (view animation)
Apoptosis
Apoptosis Overview
This animation provides a brief overview of programmed cell death. (view animation)
Intrinsic Triggering of Apoptosis
This animation provides a brief explanation of a possible intrinsic apoptotic pathway. In this case, damage to nuclear DNA triggers a cascade of events that triggers apoptosis. (view animation)
Cell-Based Assays
MultiTox-Fluor Multiplex Cytotoxicity Assay
This animation explains the advantages of a multiplexed cell-based assay for measuring cytotoxicity. (view animation)
Culture Preparation and Plating for Cell-Based Assays
This video demonstrates how to prepare and plate a culture for use in a cell-based assay. (view video)
PCR
Introduction to PCR
This animation provides a step-by-step introduction to this important molecular biology technique. (view animation)
Introduction to the Plexor™ Systems
The Plexor™ Systems are a new way to perform quantitative PCR. Understand the basic concepts behind the Plexor™ technology, then see how the Plexor™ Systems are used to quantify a single target DNA sequence or multiple target sequences. (view animation)
Genotyping with the Plexor™ qPCR System
See how the Plexor™ qPCR System can be used in genotyping assays to distinguish between two alternative bases at a specific site in a known DNA sequence. (view animation)
Cloning
pGEM®-T Vector Systems
A video protocol for the use of the pGEM®-T Vector Systems, Cat.# A1360 (view video)
Protein Expression and Analysis
Protein-Protein Interactions and the TNT® Systems
See how to use the MagneGST™ and TNT® Systems to detect protein-protein interactions between the products of cloned genes. (view animation)
Protein-DNA Interactions and the TNT® Systems
See how to use the TNT® Systems to study protein-DNA interactions in applications such as gel shift assays. (view animation)
Co-immunoprecipitation to Study Protein-Protein Interactions Using the TNT® Systems
See how to use the TNT® Systems to study protein-protein interactions using co-immunoprecipitation. (view animation)
Analysis of Protein Post-Translational Modification Using the TNT® Systems and Canine Microsomal Membranes
This animation demonstrates the analysis of protein glycosylation and signal peptide cleavage using TNT® Cell-Free Expression Systems. (view animation)
TNT® SP6 High-Yield Protein Expression System
See the advantages of the TNT® High Yield System over conventional wheat germ and E. coli systems. (view animation)
HaloCHIP™ System
Learn more about the HaloCHIP™ protocol; a ChIP assay with no antibody required. (view animation)
HaloTag® Interchangeable Labeling Technology
This animation introduces you to a specially designed fusion protein that can be covalently tagged with an interchangeable set of ligands for use in multiple applications, including cellular imaging, protein imobilization, and capture of binding proteins. (view animation)
HaloTag® Protein Purification System
The HaloTag Protein Purification System purifies proteins fused to HaloTag, a novel protein tag that enhances the expression and solubility of recombinant proteins. HaloTag Technology enables the covalent, efficient and specific capture of a protein of interest onto HaloLink Resin, thus overcoming the equilibrium-based limitations associated with affinity tags. (view animation)
HaloLink™ Protein Array Systems
A video protocol for the use of the HaloLink™ Protein Array Systems, Cat.# G6190 (view video)
Single Step (KRX) Competent Cells
Explore the features of these competent cells designed for efficient transformation of protein expression vectors and tightly controlled protein expression. (view animation)
ProteaseMAX™ Surfactant and Protein Mass Fingerprinting
Learn how ProteaseMAX™ Surfactant enhances the enzymatic performance of trypsin or chymotrypsin in any digestion reaction performed with the objective of characterizing the products by mass spectrometry or liquid chromatography. (view animation)
The Flexi® Cloning System
This animation demonstrates the use of the Flexi® Vector System for directional cloning of protein-coding sequences and the transfer of these sequences between a variety of Flexi® Vectors without the need to resequence. (view animation)
Purification
PureYield™ Plasmid Miniprep System
A video protocol for the use of the PureYield™ Plasmid Miniprep System, Cat.# A1221 (view video)
PureYield™ Plasmid Midiprep System
A video protocol for the use of the PureYield™ Plasmid Midiprep System, Cat.# A2492 (view video)
How to Use the Eluator™ Device
A video protocol for the use of the Eluator™ Device, Cat.# A1071 (view video)
Setting up a Vacuum Manifold
A video protocol for the proper assembly of a vacuum manifold. (view video)
RNA Interference
Introduction to RNA interference
This animation introduces the basic concept of RNA interference and provides an overview of DNA-directed RNA interference. (view animation)
Signal Transduction
Introduction to the MAPK Pathway
The Mitogen-Activated Protein Kinase (MAPK) signaling pathways modulate many cellular events including: the cell cycle, embryonic development, movement, apoptosis, and neuronal differentiation. (view animation)
The PI3K Pathway
This animation demonstrates some events associated with the phosphoinositol 3-kinase pathway. (view animation)