Figure 1. Assay sensitivity and linearity. Light intensity of the MAO-Glo Assay is proportional to enzyme activity over a >10,000-fold range. The signal:noise ratio was determined by dividing the net luminescent signal by the standard deviation of the background. The limit of detection is the concentration of enzyme required to generate a signal:noise ratio of 3 (green line). The MAO-Glo Assay is up to one hundred times more sensitive than competing fluorescent assays.

The MAO-Glo Assay provides a homogeneous, luminescent method for measuring monoamine oxidase (MAO) activity from recombinant and native sources and measuring the effects of test compounds on MAO activity.

Features:

• Greater Specificity: The MAO-Glo Assay is more specific for monoamine oxidase (both A and B) than assays that measure H202.
• Greater Sensitivity: Less monoamine oxidase (MAO) enzyme is required in these assays than in the typical HPLC or fluorometric methods. (Figure 1).
• No Fluorescence Interference: By using luminescence to monitor enzyme activity, any overlaps between the fluorescent excitation and emission wavelengths of test compounds are inconsequential. In fluorescent assays, such overlaps can confound analysis and present misleading or irrelevant data.