TNT® SP6 High-Yield Protein Expression System
The TNT SP6 High-Yield Protein Expression System, based on an optimized wheat germ extract is the only true coupled transcription/translation system that allows synthesis of large amounts of soluble protein (10-100µg/ml) in only two hours. This system offers many benefits:
Save time - Express protein in just two hours.
Versatility - Express protein using either plasmid or PCR generated templates.
Achieve High Yields - Attain 10-100µg/ml protein.
Animation - Introduction to the TNT
High-Yield
Protein Expression System.
Save Time
Express proteins in only two hours when using this DNA-based, cell-free system. Provided in a single-tube format, the DNA template is added directly to the Master Mix, incubated for two hours and is ready for use in a variety of applications. No additional purification is required.
Time comparison of TNT SP6 High-Yield Protein Expression System
vs. E. coli .
Versatility
Express protein using either plasmid or PCR generated templates.
Achieve High Yields
With this system, you can attain 10-100µg/ml of protein which can be used directly for faster screening of multiple targets, as well as in expanded applications. More protein also allows easier adaptation for high-throughput experiments.
Comparison of firefly luciferase protein expression profiles using various
TNT® Systems.
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