Subcloning is a basic molecular biology procedure used to move inserts from one vector to another. Essentially all subcloning reactions proceed as follows: You release and purify your insert from the parent vector, ligate the insert into a prepared destination vector, transform the ligation reaction into competent bacterial cells, then screen the transformed cells for the insert. The Subcloning Notebook will lead you through every step in this process.

See the Table of Contents below to download and view individual chapters in .pdf.

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