Restriction Enzymes Resource

3.9 Restriction Enzyme Recognition Sequences Containing Start or Stop Codons

Table 3.9A lists enzymes whose recognition sequences contain the start codon AUG. Those enzymes having a limited degree of degeneracy for the AUG codon (e.g., R, Y, W, etc.) are listed, however those that would require the AUG to be composed completely of unspecified nucleotides (e.g., NNN) are not included. These enzymes can be useful for creating promoter fusions in gene expression studies. Promega enzymes are in bold.

Table 3.9A. Enzymes that have the Start Codon (AUG) as Part of Their Recognition Sequence.

Table 3.9B lists enzymes whose recognition sequences contain the stop codons UAA, UAG and UGA. Those enzymes having a limited degree of degeneracy for the stop codons (e.g. R, Y, W, etc.) are listed, however those that would require the stop codons to be composed completely of unspecified nucleotides (e.g., NNN) are not included. Promega enzymes are in bold.

Table 3.9B. Enzymes that Have a Stop Codon (UAA, UAG, UGA) as Part of Their Recognition Sequence.

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