Restriction Enzymes Resource

3.6 Methylation Sensitivity of Isoschizomer/Neoschizomer Pairs

Heat-inactivation of restriction enzymes may be performed when a subsequent reaction can be performed in the same reaction buffer, or when the reaction will be diluted for the next application. This will eliminate the need for extra ethanol precipitations or clean-up steps. Table 3.4 lists the sensitivity of Promega's restriction enzymes to heat-inactivation.

Isoschizomers and neoschizomers often differ in their sensitivity to methylation. This can be a simple way to differentiate the methylation state of DNA. Iso/neoschizomer pairs sold by Promega are listed below.