3.3 Composition of Promega Restriction Enzyme Reaction Buffers (1X)
Promega's Restriction Enzyme Reaction Buffers are provided as 10X stock
solutions. Table
3.3 provides compositions for Restriction Enzyme Reaction Buffers, listed as 1X
concentrations.
| Buffer |
pH
(at 37°C) |
Tris-HCl
(mM) |
MgCl2
(mM) |
NaCl
(mM) |
KCl
(mM) |
DTT
(mM) |
| A |
7.5 |
6 |
6 |
6 |
-
|
1 |
| B |
7.5 |
6 |
6 |
50 |
-
|
1 |
| C |
7.9 |
10 |
10 |
50 |
-
|
1 |
| D |
7.9 |
6 |
6 |
150 |
-
|
1 |
| E |
7.5 |
6 |
6 |
100 |
-
|
1 |
| F |
8.5 |
10 |
10 |
100 |
-
|
1 |
| G |
8.2 |
50 |
5 |
-
|
-
|
-
|
| H |
7.5 |
90 |
10 |
50 |
-
|
-
|
| J |
7.5 |
10 |
7 |
-
|
50 |
1 |
| K |
7.4 |
10 |
10 |
-
|
150 |
-
|
| L |
9.0 |
10 |
3 |
100 |
-
|
-
|
| MULTI-CORE™ Buffer (1X) = 25mM Tris-Acetate, pH 7.5 (at 37°C), 100mM potassium
acetate, 10mM magnesium acetate, 1mM DTT. Notes:
- For each 10°C rise in temperature between 0°C and
25°C, the pH of Tris buffers decreases 0.31 pH units.
- For each 10°C rise in temperature between 25°C and
37°C, the pH of Tris buffers decreases 0.25 pH units.
|
|
