3.2 Activity of Cloning Enzymes in Buffer H.
EcoR I and Pst I are commonly used enzymes in double digests and cloning
applications. Their optimal buffer is H. Table 3.2 provides the relative activity of other
cloning enzymes in Buffer H for double digest purposes.
|
Aat II
|
<10% |
|
Mlu I |
100-125%* |
| Acc I |
<10% |
|
Nco I |
100-125%* |
| Acc65 I |
100-125%* |
|
Nhe I |
10-25% |
| Apa I |
<10% |
|
Not I |
100-125%* |
| Ava I |
10-25% |
|
Nsi I |
>125%* |
| BamH I |
50-75% |
|
Pst I |
100% |
| Bbu I |
10-25% |
|
Sac I |
25-50% |
| Bcl I |
50-75% |
|
Sac II |
>125%* |
| Bgl I |
75-100% |
|
Sal I |
25-50% |
| BstX I |
75-100% |
|
Sfi I |
50-75% |
| BstZ I |
75-100% |
|
Sma I |
<10% |
| Cla I |
50-75% |
|
Spe I |
25-50% |
| Csp I |
100-125%* |
|
Sph I |
>125%* |
| Csp45 I |
25-50% |
|
Spo I |
<10% |
| Eco52 I |
50-75% |
|
Ssp I |
100-125%* |
| EcoR I |
100% |
|
Sty I |
50-75% |
| EcoR V |
50-75% |
|
Xba I |
100-125%* |
| Hinc II |
50-75% |
|
Xho I |
100-125%* |
| Hind III |
25-50% |
|
Xma I |
<10% |
| Kpn I |
<10% |
|
|
|
|
