Table 3.1 Relative Activity of Restriction Enzymes in
Promega's 10X Buffers
The 10X Reaction Buffer supplied with each restriction enzyme is optimized to give 100%
activity. In many cases, good activity is also obtained using one of Promega's 4-CORE®
10X Buffers (Cat.# R9921).
Table 3.1 may be used to select the best buffer for digestions with multiple restriction
enzymes. Enzyme activity is expressed as a percent of the activity obtained with the
optimized buffer for each enzyme.
Promega
Enzyme |
Buffer Supplied with Enzyme |
4 CORE® Buffers |
MULTI-
CORE™
Buffer |
Enzyme Assay
Temp. |
| A |
B |
C |
D |
| Aat II |
J |
50-75% |
10-25% |
<10% |
<10% |
<10% |
37°C |
| Acc I |
G |
50-75% |
25-50% |
25-50% |
10-25% |
25-50% |
37°C |
| Acc III |
F |
<10% |
10-25% |
25-50% |
25-50% |
<10% |
65°C |
| Acc65 I |
D |
10-25% |
50-75% |
75-100% |
100% |
100% |
37°C |
| AccB7 I |
E |
10-25% |
50-75% |
100%** |
<10% |
100% |
37°C |
| Age I |
K |
25-50% |
25-50% |
25-50% |
50-75% |
100% |
37°C |
| Alu I |
B |
75-100% |
100% |
75-100% |
10-25% |
10-25% |
37°C |
| Alw26 I |
C |
10-25% |
25-50% |
100% |
10-25% |
75-100% |
37°C |
| Alw44 I |
C |
<10% |
25-50% |
100% |
25-50% |
100% |
37°C |
| Apa I |
A |
100% |
50-75% |
50-75% |
<10% |
75-100% |
37°C |
| Ava I |
B |
10-25% |
100% |
50-75% |
25-50% |
<10% |
37°C |
| Ava II |
C |
50-75% |
50-75% |
100% |
25-50% |
25-50% |
37°C |
| Bal I |
G |
10-25% |
<10% |
<10% |
<10% |
<10% |
37°C |
| BamH I |
E |
75-100% ** |
75-100% |
75-100% |
50-75% |
75-100% |
37°C |
| Ban I |
G |
25-50% |
25-50% |
10-25% |
<10% |
100% |
50°C |
| Ban II |
E |
75-100% |
75-100% |
75-100% |
25-50% |
100% |
37°C |
| Bbu I |
A |
100% |
75-100% |
75-100% |
<10% |
100% |
37°C |
| Bcl I |
C |
10-25% |
75-100% |
100% |
50-75% |
10-25% |
50°C |
| Bgl I |
D |
10-25% |
25-50% |
75-100% |
100% |
100% |
37°C |
| Bgl II |
D |
25-50% |
75-100% |
75-100% |
100% |
<10% |
37°C |
| BsaM I |
D |
10-25% |
25-50% |
50-75% |
100% |
25-50% |
65°C |
| BsaO I |
C |
10-25% |
50-75% |
100% |
25-50% |
100% |
50°C |
| Bsp
I286 I |
A |
100% |
50-75% |
25-50% |
10-25% |
75-100% |
37°C |
| BsrBR I |
H |
10-25% |
50-75%** |
100%** |
50-75% |
100% |
65°C |
| BsrS I |
D |
10-25% |
25-50% |
10-25% |
100% |
100% |
65°C |
|
-top
of page-
|
Promega
Enzyme |
Buffer Supplied
with Enzyme |
4 CORE® Buffers |
MULTI-
CORE™ Buffer |
Enzyme Assay
Temperature |
| A |
B |
C |
D |
| BssH II |
H |
75-100% |
50-75% |
75-100% |
50-75% |
75-100% |
50°C |
| Bst71 I |
D |
10-25% |
25-50% |
25-50% |
100% |
10-25% |
50°C |
| Bst98 I |
D |
<10% |
10-25% |
10-25% |
100% |
25-50% |
37°C |
| BstE II |
D |
25-50% |
50-75% |
50-75% |
100% |
100% |
60°C |
| BstO I |
C |
10-25% |
25-50% |
100% |
25-50% |
<10% |
60°C |
| BstX I |
D |
<10% |
10-25% |
25-50% |
100% |
10-25% |
50°C |
| BstZ I |
D |
<10% |
<10% |
10-25% |
100% |
10-25% |
50°C |
| Bsu36 I |
E |
<10% |
25-50% |
50-75% |
25-50% |
50-75% |
37°C |
| Cfo I |
B |
75-100% |
100% |
75-100% |
25-50% |
100% |
37°C |
| Cla I |
C |
75-100% |
75-100% |
100% |
75-100% |
100% |
37°C |
| Csp I |
K |
<10% |
10-25% |
25-50% |
50-75% |
10-25% |
30°C |
| Csp45 I |
B |
25-50% |
100% |
50-75% |
25-50% |
50-75% |
37°C |
| Dde I |
D |
25-50% |
25-50% |
50-75% |
100% |
25-50% |
37°C |
| Dpn I |
B |
50-75% |
100% |
75-100% |
50-75% |
100% |
37°C |
| Dra I |
B |
75-100% |
100% |
75-100% |
50-75% |
25-50% |
37°C |
| EclHK I |
E |
<10% |
<10% |
75-100% |
10-25% |
50-75% |
37°C |
| Eco47 III |
D |
<10% |
25-50% |
50-75% |
100% |
25-50% |
37°C |
| Eco52 I |
L |
<10% |
<10% |
10-25% |
25-50% |
<10% |
37°C |
| EcoICR I |
B |
10-25% |
100% |
75-100% |
<10% |
100% |
37°C |
| EcoR I |
H |
25-50% |
50-75% |
50-75% |
50-75% |
100% |
37°C |
| EcoR V |
D |
10-25% |
25-50% |
50-75% |
100% |
100% |
37°C |
| Fok I |
B |
75-100% |
100% |
75-100% |
25-50% |
50-75% |
37°C |
| Hae II |
B |
50-75% |
100% |
50-75% |
10-25% |
100% |
37°C |
| Hae III |
C |
75-100% |
75-100% |
100% |
50-75% |
100% |
37°C |
|
-top
of page-
|
Promega
Enzyme |
Buffer Supplied
with Enzyme |
4 CORE® Buffers |
MULTI-
CORE™ Buffer |
Enzyme Assay
Temperature |
| A |
B |
C |
D |
| Hha I |
C |
50-75% |
75-100% |
100% |
50-75% |
75-100% |
37°C |
| Hinc II |
B |
25-50% |
100% |
25-50% |
50-75% |
100% |
37°C |
| Hind III |
E |
25-50% |
100% |
75-100% |
10-25% |
50-75% |
37°C |
| Hinf I |
B |
50-75% |
100% |
75-100% |
75-100% |
50-75% |
37°C |
| Hpa I |
J |
25-50% |
50-75% |
25-50% |
10-25% |
100% |
37°C |
| Hpa II |
A |
100% |
50-75% |
50-75% |
10-25% |
100% |
37°C |
| Hsp92 I |
F |
10-25% |
75-100% |
50-75% |
25-50% |
10-25% |
37°C |
| Hsp92 II |
K |
10-25% |
25-50% |
25-50% |
<10% |
<10% |
37°C |
| I-Ppo I |
I-Ppo I |
10-25% |
25-50% |
25-50% |
25-50% |
- |
37°C |
| Kpn I |
J |
100%** |
25-50% |
25-50% |
<10% |
75-100% |
37°C |
| Mbo I |
C |
10-25% |
75-100% |
100% |
50-75% |
<10% |
37°C |
| Mbo II |
B |
10-25% |
100% |
50-75% |
75-100% |
100% |
37°C |
| Mlu I |
D |
10-25% |
25-50% |
50-75% |
100% |
10-25% |
37°C |
| Msp I |
B |
75-100% |
100% |
75-100% |
25-50% |
25-50% |
37°C |
| MspA1 I |
C |
25-50% |
100%** |
100% |
10-25% |
100% |
37°C |
| Nae I |
A |
100% |
50-75% |
25-50% |
<10% |
50-75% |
37°C |
| Nar I |
G |
75-100% |
50-75% |
75-100% |
25-50% |
50-75% |
37°C |
| Nci I |
B |
100%** |
100% |
25-50% |
25-50% |
50-75% |
37°C |
| Nco I |
D |
50-75% |
75-100% |
75-100% |
100% |
75-100% |
37°C |
| Nde I |
D |
<10% |
<10% |
25-50% |
100% |
25-50% |
37°C |
| Nde II |
D |
<10% |
<10% |
10-25% |
100% |
25-50% |
37°C |
| NgoM IV |
MULTI-
CORE™ |
100%** |
100%** |
100%** |
<10% |
100% |
37°C |
| Nhe I |
B |
75-100% |
100% |
75-100% |
10-25% |
100% |
37°C |
| Not I |
D |
<10% |
10-25% |
25-50% |
100% |
25-50% |
37°C |
|
-top
of page-
|
Promega
Enzyme |
Buffer Supplied
with Enzyme |
4 CORE® Buffers |
MULTI-
CORE™ Buffer |
Enzyme Assay
Temperature |
| A |
B |
C |
D |
| Nru I |
K |
<10% |
<10% |
<10% |
50-75% |
10-25% |
37°C |
| Nsi I |
D |
10-25% |
50-75% |
50-75% |
100% |
10-25% |
37°C |
| Pst I |
H |
10-25% |
50-75% |
50-75% |
50-75% |
25-50% |
37°C |
| Pvu I |
D |
10-25% |
25-50% |
50-75% |
100% |
<10% |
37°C |
| Pvu II |
B |
25-50% |
100% |
50-75% |
25-50% |
50-75% |
37°C |
| Rsa I |
C |
75-100% |
75-100% |
100% |
<10% |
<10% |
37°C |
| Sac I |
J |
75-100% |
25-50% |
25-50% |
<10% |
100% |
37°C |
| Sac II |
C |
100%* |
50-75% |
100% |
50-75% |
<10% |
37°C |
| Sal I |
D |
<10% |
10-25% |
25-50% |
100% |
<10% |
37°C |
| Sau3A I |
B |
25-50% |
100% |
75-100% |
<10% |
100% |
37°C |
| Sau96 I |
C |
25-50% |
25-50% |
100% |
50-75% |
50-75% |
37°C |
| Sca I |
K |
<10% |
100%** |
50-75% |
75-100% |
10-25% |
37°C |
| Sfi I |
B |
75-100% |
100% |
75-100% |
25-50% |
75-100% |
50°C |
| Sgf I (a) |
C |
25-50% |
| 