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Restriction Enzymes Resource

Table 1.6 Restriction Enzyme Quality Control Specifications

Enzyme

Recognition Sequence

Substrate Used

Plasmid for B/W Cloning Assay

 Assay Temperature QC Specifications
Activity OD C/L/R Activity Ligation Low Conc. (u/µl) % Ligation / Recut OD Units
(16hr)
Sac I GAGCT / C EcoR I
fragment		 EcoR I
fragment		 Ad-2 3Zf, 5Zf, 7Zf, 9Zf 37°C 16°C/2 hours 10 90/90 > 20
Sac I (HC) GAGCT / C EcoR I
fragment		 EcoR I
fragment		 Ad-2 3Zf, 5Zf, 7Zf, 9Zf 37°C 16°C/2 hours 40-80 90/90 > 20
Sac II CCGC / GG Ad-2 lambda Ad-2 5ZF 37°C 4°C/16 hours 10 90/90 > 20
Sac II (HC) CCGC / GG Ad-2 lambda Ad-2 5ZF 37°C 4°C/16 hours 40-80 90/90 > 20
Sal I G / TCGAC Ad-2 Ad-2 Ad-2 3Zf, 5Zf, 9Zf, 11Zf 37°C 16°C/16 hours 10 90/90 > 50
Sal I (HC) G / TCGAC Ad-2 Ad-2 Ad-2 3Zf, 5Zf, 9Zf, 11Zf 37°C 16°C/16 hours 40-80 90/90 > 50
Sau3A I / GATC lambda lambda lambda NA 37°C 4°C/16 hours 3-10 90/90 > 10
Sau96 I G / GNCC lambda lambda lambda NA 37°C 16°C/16 hours 10 90/90 > 10
Sca I AGT / ACT lambda lambda pBR322 NA 37°C 16°C/16 hours 8-12 90/90 > 20
Sca I (HC) AGT / ACT lambda lambda pBR322 NA 37°C 16°C/16 hours 40-80 90/90 > 20
Sfi I GGCCN (4) / NGGCC Ad-2 Ad-2 pGEM®-13Zf 5Zf, 11Zf, 13Zf 50°C 16°C/16 hours 10 90/90 > 20
Sfi I (HC) GGCCN (4) / NGGCC Ad-2 Ad-2 pGEM®-13Zf 5Zf, 11Zf, 13Zf 50°C 16°C/16 hours 40-80 90/90 > 20
Sgf I(a) GCGAT / CGC Ad-2 Ad-2 Ad-2 NA 37°C 4°C/16 hours 8-12 50/90 > 5
Sgf I (HC) GCGAT / CGC Ad-2 Ad-2 Ad-2 NA 37°C 4°C/16 hours 40-80 50/90 > 5
Sin I G / G(W)CC lambda lambda lambda NA 37°C 16°C/2 hours 8-12 90/90 > 20
Sin I (HC) G / G(W)CC lambda lambda lambda NA 37°C 16°C/2 hours 40-80 90/90 > 40
Sma I CCC / GGG lambda lambda pGEM®-3Zf 3Zf, 7Zf 25°C 16°C/2 hours 8-12 90/90 > 50
Sma I (HC) CCC / GGG lambda lambda pGEM®-3Zf 3Zf, 7Zf 25°C 16°C/2 hours 40-80 90/90 > 100
SnaB I TAC / GTA T7 lambda T7 NA 37°C 16°C/16 hours 2-10 90/80 > 8
Spe I A / CTAGT Ad-2 Ad-2 Ad-2 5Zf, 9Zf 37°C 4°C/16 hours 10 90/90 > 20
Sph I GCATG / C lambda lambda lambda 3Zf, 5Zf, 7Zf, 11Zf 37°C 4°C/16 hours 10 90/90 > 50
Ssp I AAT / ATT lambda lambda pBR322 NA 37°C 4°C/16 hours 10 90/90 > 10
Ssp I (HC) AAT / ATT lambda lambda pBR322 NA 37°C 4°C/16 hours 40-80 90/90 > 10
Stu I AGG / CCT lambda lambda Ad-2 NA 37°C 4°C/16 hours 10 90/90 > 50
Sty I C / C(W)(W)GG lambda lambda lambda 5Zf 37°C 16°C/16 hours 10 90/90 > 40
Taq I T / CGA lambda lambda lambda NA 65°C 16°C/2 hours 10 90/90 > 50
Taq I (HC) T / CGA lambda lambda lambda NA 65°C 16°C/2 hours 40-80 90/90 > 80
Tth111 I GACN / NNGTC Ad-2 Ad-2 lambda NA 65°C 16°C/2 hours 8-12 0/0 > 5
Tru9 I T / TAA lambda lambda lambda NA 65°C 16°C/2 hours 8-12 90/90 > 100
Vsp I AT / TAAT lambda lambda lambda NA 37°C 16°C/16 hours 8-12 70/90 > 50
Xba I T / CTAGA Ad-2 Ad-2 Ad-2 3Zf, 7Zf, 9Zf, 11Zf 37°C 16°C/2 hours 8-12 90/90 > 50
Xba I (HC) T / CTAGA Ad-2 Ad-2 Ad-2 3Zf, 7Zf, 9Zf, 11Zf 37°C 16°C/2 hours 40-80 90/90 > 80
Xho I C / TCGAG EcoR I
fragment		 lambda phiX174 7Zf, 11Zf 37°C 16°C/16 hours 10 90/90 > 100
Xho I (HC) C / TCGAG EcoR I
fragment		 lambda phiX174 7Zf, 11Zf 37°C 16°C/16 hours 40-80 90/90 > 100
Xho II (R) / GATC(Y) pBR322 phiX174 lambda NA 37°C 16°C/16 hours 5-10 90/90 > 100 (phiX174)
pBR322 37°C > 80 (pBR322)
Xma I C / CCGGG Ad-2 Ad-2 Ad-2 3Zf 37°C 16°C/2 hours 1-5 90/90 > 5
Xmn I GAANN / NNTTC lambda lambda lambda NA 37°C 4°C/16 hours 10 80/95 > 5
HC = High Concentration.
NA = Not Applicable.

(a)U.S. Pat. No. 5,391,487 has been issued to Promega Corporation for Restriction Endonuclease Sgf I.

Additional Enzymes:
Aat to Bgl | Bsa to Csp | Dde to I-Ppo | Kpn to Rsa | Sac to Xmn

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