RE Resource, Table 1.6

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Restriction Enzymes Resource

Table 1.6 Restriction Enzyme Quality Control Specifications

Enzyme	Recognition Sequence	Substrate Used			Plasmid for B/W Cloning Assay	Assay Temperature		QC Specifications
Enzyme	Recognition Sequence	Activity	OD	C/L/R	Plasmid for B/W Cloning Assay	Activity	Ligation	Low Conc. (u/µl)	% Ligation / Recut	OD Units (16 hours)
Kpn I	GGTAC / C	Ad-2	Ad-2	EcoR I fragment	3Zf, 7Zf	37°C	16°C/2 hours	8-12	90/90	> 10
Kpn I (HC)	GGTAC / C	Ad-2	Ad-2	EcoR I fragment	3Zf, 7Zf	37°C	16°C/2 hours	40-80	90/90	> 10
Mbo I	/ GATC	lambda*	lambda*	lambda*	NA	37°C	16°C/2 hours	8-12	95/95	> 30
Mbo II	GAAGAN (8/7)	lambda*	lambda*	lambda*	NA	37°C	16°C/16 hours	8-12	20/90	>= conc
Mlu I	A / CGCGT	lambda	lambda	lambda	5Zf, 7Zf	37°C	16°C/16 hours	10	90/90	> 20
Msp I	C / CGG	lambda	lambda	lambda	NA	37°C	4°C/16 hours	10	90/90	> 100
Msp I (HC)	C / CGG	lambda	lambda	lambda	NA	37°C	4°C/16 hours	40-80	90/90	> 100
MspAl I	C(M)G / C(K)G	lambda	lambda	lambda	NA	37°C	16°C/16 hours	10	90/90	> 10
Nae I	GCC / GGC	Ad-2	Ad-2	Ad-2	NA	37°C	16°C/16 hours	10	90/90	>5
Turbo™ Nae I	GCC / GGC	Ad-2	Ad-2	Ad-2	NA	37°C	16°C/16 hours	10	90/90	(>5)
Nar I	GG / CGCC	Ad-2	Ad-2	Ad-2	NA	37°C	16°C/16 hours	10	90/90	> 50
Turbo™ Nar I	GG / CGCC	Ad-2	Ad-2	Ad-2	NA	37°C	16°C/16 hours	10	90/90	(>5)
Nci I	CC / (S)GG	lambda	lambda	lambda	NA	37°C	16°C/2 hours	10	30/90	> 30
Nco I	C / CATGG	lambda	lambda	lambda	5Zf	37°C	16°C/16 hours	10	90/90	> 80
Nco I (HC)	C / CATGG	lambda	lambda	lambda	5Zf	37°C	16°C/16 hours	40-80	90/90	> 80
Nde I	CA / TATG	lambda	lambda	lambda	NA	37°C	16°C/2 hours	10	90/90	> 50
Nde II	/ GATC	lambda*	lambda*	lambda*	NA	37°C	16°C/2 hours	10	90/90	> 50
NgoM IV	G / CCGGC	Ad-2	Ad-2	Ad-2	NA	37°C	16°C/2 hours	10	90/90	> 50
Nhe I	G / CTAGC	EcoR I fragment	Ad-2	Ad-2	NA	37°C	16°C/16 hours	10	90/90	> 20
Nhe I (HC)	G / CTAGC	EcoR I fragment	Ad-2	Ad-2	NA	37°C	16°C/16 hours	40-80	90/90	> 20
Not I	GC / GGCCGC	Ad-2	Ad-2	Ad-2	5Zf, 9Zf, 11Zf, 13Zf	37°C	16°C/2 hours	10	90/90	> 80
Not I (HC)	GC / GGCCGC	Ad-2	Ad-2	Ad-2	5Zf, 9Zf, 11Zf, 13Zf	37°C	16°C/2 hours	40-80	90/90	> 80
Nru I	TCG / CGA	lambda	lambda	lambda	NA	37°C	16°C/16 hours	10	20/90	> 40
Nsi I	ATGCA / T	lambda	lambda	lambda	5Zf, 7Zf, 9Zf, 11Zf	37°C	16°C/16 hours	10	90/90	> 40
Pst I	CTGCA / G	lambda	lambda	lambda	3Zf, 5Zf	37°C	16°C/2 hours	10	90/90	> 50
Pst I (HC)	CTGCA / G	lambda	lambda	lambda	3Zf, 5Zf	37°C	16°C/2 hours	40-80	90/90	> 50
Pvu I	CGAT / CG	lambda	lambda	Ad-2	NA	37°C	16°C/16 hours	2-10	90/90	> 10
Pvu II	CAG / CTG	lambda	lambda	lambda	NA	37°C	4°C/16 hours	8-12	90/90	> 10
Rsa I	GT / AC	lambda	lambda	lambda	NA	37°C	16°C/16 hours	10	90/90	> 100
Rsa I (HC)	GT / AC	lambda	lambda	lambda	NA	37°C	16°C/16 hours	40-80	90/90	> 100

HC = High Concentration.
NA = Not Applicable.
*Unmethylated DNA.

Turbo is a trademark of Promega Corporation.

Additional Enzymes:
Aat to Bgl | Bsa to Csp | Dde to I-Ppo | Kpn to Rsa | Sac to Xmn

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