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Restriction Enzymes Resource

Table 1.6 Restriction Enzyme Quality Control Specifications

 

Enzyme

  

Recognition Sequence

Substrate Used

  

Plasmid for B/W Cloning Assay

 Assay Temperature QC Specifications
Activity OD C/L/R Activity Ligation Low Conc. (u/µl) % Ligation / Recut OD Units
(16 hours)
Dde I C / TNAG lambda lambda lambda NA 37°C 16°C/16 hours 8-12 90/90 > 50
Dpn I G(meth)A / TC pBR322 pBR322 pBR322 NA 37°C 16°C/16 hours 10 80/90 > 10
Dra I TTT / AAA lambda lambda lambda NA 37°C 16°C/16 hours 10 80/90 > 50
EclHK I GACNNN / NNGTC lambda lambda lambda NA 37°C 16°C/2 hours 10 10/90 > 30
Eco47 III AGC / GCT lambda lambda pBR322 NA 37°C 4°C/16 hours 2-5 80/90 > 10
Eco52 I C / GGCCG EcoR I
fragment		 lambda lambda 9Zf, 13Zf 37°C 16°C/16 hours 1-5 90/90 > 30
EcoICR I GAG / CTC EcoR I
fragment		 EcoR I
fragment		 Ad-2 3Zf, 5Zf, 7Zf, 9Zf 37°C 16°C/16 hours 10 90/95 > 50
EcoICR I (HC) GAG / CTC EcoR I
fragment		 EcoR I
fragment		 Ad-2 3Zf, 5Zf, 7Zf, 9Zf 37°C 16°C/16 hours 40-80 90/95 > 50
EcoR I G / AATTC lambda lambda lambda 3Zf, 7Zf, 9Zf, 11Zf 37°C 16°C/2 hours 8-12 90/90 > 12
EcoR I (HC) G / AATTC lambda lambda lambda 3Zf, 7Zf, 9Zf, 11Zf 37°C 16°C/2 hours 40-80 90/90 > 12
EcoR V GAT / ATC lambda lambda lambda 5Zf 37°C 4°C/16 hours 10 90/90 > 40
EcoR V (HC) GAT / ATC lambda lambda lambda 5Zf 37°C 4°C/16 hours 40-80 90/90 > 100
Fok I GGATGN (9 / 13) lambda lambda lambda NA 37°C 4°C/16 hours 2-10 90/90 > 2
Hae II (R)GCGC / (Y) lambda lambda lambda NA 37°C 16°C/16 hours 10 90/90 > 20
Hae III GG / CC lambda lambda lambda NA 37°C 16°C/2 hours 8-12 90/90 > 100 (lambda)
Hae III (MC) GG / CC lambda phiX174 lambda NA 37°C 16°C/2 hours 10-20 90/90 > 100 (phiX174)
Hae III (HC) GG / CC lambda phiX174 lambda NA 37°C 16°C/2 hours 40-80 90/90 > 100 (phiX174)
Hha I GCG / C lambda lambda lambda NA 37°C 16°C/2 hours 10 90/90 > 40
Hinc II GT(Y) / (R)AC lambda lambda lambda 3Zf, 5Zf, 9Zf, 11Zf 37°C 4°C/16 hours 10 80/90 > 20
Hinc II (HC) GT(Y) / (R)AC lambda lambda lambda 3Zf, 5Zf, 9Zf, 11Zf 37°C 4°C/16 hours 40-80 80/90 > 20
Hind III A / AGCTT lambda lambda lambda 3Zf, 7Zf, 9Zf, 11Zf 37°C 16°C/2 hours 10 90/90 > 50
Hind III (HC) A / AGCTT lambda lambda lambda 3Zf, 7Zf, 9Zf, 11Zf 37°C 16°C/2 hours 40-80 90/90 > 50
Hinf I G / ANTC lambda lambda lambda NA 37°C 16°C/16 hours 10 90/90 > 30
Hinf I (HC) G / ANTC lambda lambda lambda NA 37°C 16°C/16 hours 40-80 90/90 > 100
Hpa I GTT / AAC lambda lambda SV40 NA 37°C 16°C/16 hours 3-10 80/90 > 5
Hpa II C / CGG lambda lambda lambda NA 37°C 16°C/2 hours 10 90/90 > 80
Hsp92 I G(R) / CG(Y)C lambda lambda lambda NA 37°C 16°C/2 hours 10 90/90 >90
Hsp92 II CATG / lambda lambda lambda NA 37°C 4°C/16 hours 10 90/90 > 80
I-Ppo I CTCTCTTAA / GGTAGC I-Ppo I I-Ppo I I-Ppo I NA 37°C 4°C/16 hours 100-200 90/90 > 200

HC = High Concentration.
MC = Medium Concentration.
NA = Not Applicable.

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