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Restriction Enzymes Resource 

Table 1.6 Restriction Enzyme Quality Control Specifications

Enzyme

Recognition Sequence

Substrate Used

Plasmid for B/W Cloning Assay

 Assay Temperature QC Specifications
Activity OD C/L/R Activity Ligation Low Conc. (u/µl) % Ligation / Recut OD Units
(16hr)
BsaM I GAATGCN / lambda lambda lambda NA 65°C 4°C/16 hours 10 90/90 > 40
BsaO I CG(R)(Y) / CG lambda lambda lambda NA 50°C 4°C/16 hours 10 95/90 > 10
Bsp1286 I G(D)GC(H) / C lambda lambda lambda NA 37°C 16°C/16 hours 10 85/85 > 15
BsrBR I GATNN / NNATC lambda lambda lambda NA 65°C 16°C/16 hours 10 90/90 >100
BsrS I ACTGGN / phiX174 phiX174 lambda NA 65°C 4°C/16 hours 10 90/90 > 100
BssH II G / CGCGC lambda lambda lambda NA 50°C 16°C/16 hours 10 95/95 > 60
Bst71 I GCAGCN (8 / 12) lambda lambda lambda NA 50°C 4°C/16 hours 5-10 90/90 > 5
Bst98 I C/TTAAG Ad-2 Ad-2 PhiX174 NA 37°C 16°C/16 hours 8-12 90/90 > 100
BstE II G / GTNACC lambda lambda lambda NA 60°C 4°C/16 hours 10 95/95 > 10
BstO I CC / (W)GG lambda lambda lambda NA 60°C 16°C/16 hours 10 0/0 > 20
BstX I CCANNNNN/NTGG lambda lambda phiX174 5Zf, 7Zf 50°C 16°C/16 hours 8-12 90/90 > 20
BstZ I C / GGCCG EcoR I fragment EcoR I fragment lambda 9Zf, 13Zf 50°C 16°C/16 hours 10 90/90 > 100
Bsu36 I CC / TNAGG lambda lambda Hind III fragment NA 37°C 16°C/16 hours 10 80/80 > 100
Cfo I GCG / C lambda lambda lambda NA 37°C 4°C/16 hours 10 90/90 > 100
Cla I AT / CGAT lambda lambda lambda 7Zf 37°C 4°C/16 hours 10 90/90 > 40
Csp I CG / G(W)CCG lambda lambda lambda NA 30°C 16°C/2 hours 10 90/90 > 50
Csp45 I TT / CGAA lambda lambda lambda 7Zf 37°C 4°C/16 hours 10 90/90 > 50
Csp45 I (HC) TT / CGAA lambda lambda lambda 7Zf 37°C 4°C/16 hours 40-80 90/90 > 50

HC = High Concentration.
NA = Not Applicable.

Additional Enzymes:
Aat to Bgl to Bgl | Bsa to Csp | Dde to I-Ppo | Kpn to Rsa | Sac to Xmn

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