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Restriction Enzymes Resource

Table 1.6 Restriction Enzyme Quality Control Specifications

Enzyme

Recognition Sequence

Substrate Used

Plasmid for B/W Cloning Assay

 Assay Temperature QC Specifications
Activity OD C/L/R Activity Ligation Low Conc. (u/µl) % Ligation / Recut OD Units
(16hr)
Aat II GACGT / C lambda lambda lambda 5Zf, 7Zf 37°C 4°C/16 hours 3-5 90/90 > 5
Acc I GT/(M)(K)AC lambda lambda lambda 3Zf, 5Zf, 9Zf, 11Z 37°C 4°C/16 hours 3-10 90/90 > 30
Acc III T / CCGGA lambda lambda lambda NA 65°C 4°C/16 hours 10 90/90 > 10
Acc65 I G / GTACC Ad-2 Ad-2 Ad-2 3Zf, 7Zf 37°C 4°C/16 hours 10 90/90 > 40
AccB7 I CCANNNN/ NTGG lambda lambda lambda NA 37°C 4°C/16 hours 10 90/90 > 20
Age I A / CCGGT lambda lambda lambda NA 37°C 16°C/16 hours 3-10 90/90 > 30
Alu I AG / CT lambda lambda lambda NA 37°C 16°C/16 hours 10 90/90 > 40
Alw26 I GTCTCN (1/5) lambda lambda lambda NA 37°C 16°C/16 hours 8-12 90/90 > 20
Alw44 I G / TGCAC lambda lambda lambda NA 37°C 16°C/16 hours 10 90/90 > 20
Apa I GGGCC/C Ad-2 lambda Ad-2 5Zf, 7Zf, 11Zf 37°C 4°C/16 hours 8-12 90/90 > 100
Apa I (HC) GGGCC/C Ad-2 lambda Ad-2 NA 37°C 4°C/16 hours 40-80 90/90 > 100
Ava I C / (Y)CG(R)G lambda lambda lambda 3Zf, 11Zf 37°C 16°C/16 hours 8-12 90/90 > 20
Ava II G / G(W)CC lambda* lambda* lambda* NA 37°C 16°C/16 hours 1-10 90/90 > 20
Bal I TGG / CCA lambda* lambda* lambda* NA 37°C 16°C/16 hours 2-10 80/90 > 20
BamH I G / GATCC lambda lambda lambda 3Zf, 7Zf, 11Zf 37°C 4°C/16 hours 10 90/90 >20
BamH I (HC) G / GATCC lambda lambda lambda 3Zf, 7Zf, 11Zf 37°C 4°C/16 hours 40-80 90/90 >20
Ban I G / G(Y)(R)CC lambda lambda lambda NA 50°C 16°C/2 hours 8-12 90/90 > 10
Ban II G(R)GC(Y) / C lambda lambda lambda NA 37°C 4°C/16 hours 8-12 90/90 > 15
Bbu I GCATG / C lambda lambda lambda NA 37°C 16°C/2 hours 10 90/90 > 20
Bbu I (HC) GCATG / C lambda lambda lambda NA 37°C 16°C/2 hours 40-80 90/90 > 20
Bcl I T / GATCA lambda* lambda* lambda* NA 50°C 16°C/16 hours 10 90/90 > 5
Bcl I (HC) T / GATCA lambda* lambda* lambda* NA 50°C 16°C/16 hours 40-80 90/90 > 5
Bgl I GCCNNNN / NGGC lambda lambda SV40 NA 37°C 16°C/16 hours 10 90/90 > 40
Bgl I (HC) GCCNNNN / NGGC lambda lambda SV40 NA 37°C 16°C/16 hours 40-80 90/90 > 40
Bgl II A / GATCT lambda lambda lambda NA 37°C 16°C/2 hours 10 90/90 > 25
Bgl II (HC) A / GATCT lambda lambda lambda NA 37°C 16°C/2 hours 40-80 90/90 > 25

HC = High Concentration.
NA = Not Applicable.
*Unmethylated DNA.

Additional Enzymes:
Aat to Bgl | Bsa to Csp | Dde to I-Ppo | Kpn to Rsa | Sac to Xmn

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