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Restriction Enzymes Resource
Restriction enzymes (REs) that are
most useful for molecular biology applications possess two essential
attributes: high sequence specificity
& precision cutting. Our Restriction Enzymes
Resource is an interactive tool, designed to speed your research
applications, for identifying REs and RE recognition
sites. We offer four
searches, described immediately below, as well as background
& reference information on restriction enzymes.
Searches
| SEARCH BY |
|
DESCRIPTION |
| Enzyme Name |
|
Find information
on recognition site, cut site, isoschizomer(s), neoschizomer(s) and type of overhang. |
| Recognition Sequence |
|
Identify REs that recognize a specific nucleotide sequence. |
| Enzymes Generating Compatible
Ends |
|
Find compatible REs by the type of end generated (overhang or
blunt) and overhang sequence. |
| Compatible Buffers
|
|
Identify a reaction buffer for simultaneous, multiple restriction digests
(Promega enzymes only). |
Background & Reference
Information
The following sections of the Restriction Enzymes Resource contain general information relevant to
all restriction enzymes, as well as functional groupings of enzymes with particular characteristics,
procedures and applications for use, and enzyme-specific technical data. The
resource provides
both background information and technical details on the use of restriction enzymes in
current applications. For many purposes, more than one restriction enzyme may yield an
acceptable product. This resource assists researchers in choosing the
appropriate enzyme for each application.
Table
of Contents
- General Information
- Applications and Reaction Conditions
- Reference Information
- Online Searches
Glossary
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