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Guides

Protein Interactions 

 Protein Interaction Guide, Promega
Complete Guide (.pdf, 999kb)

 

Protein interactions are critical to the majority of cellular processes, including replication, transcription, translation and signal transduction. The characterization of these interactions aids researchers in determining the function of proteins and the genes that encode them.

This guide provides an overview of common techniques used for protein interaction analysis and will assist you in determining which individual technique or combination of techniques is best suited for a particular situation/experiment.

The guide is available in .pdf.  Select the cover, to the left, to download the entire guide, or use the Table of Contents, below, to select an individual chapter.

If you have any difficulty accessing the file, please consult our Portable Document Format page.

Table of Contents
1 Yeast Two-hybrid System (.pdf, 139kb)
2 Mammalian Two-hybrid System (.pdf, 137kb)
3 Pull-down Assays (.pdf, 245kb)
4 Co-immunoprecipitation (.pdf, 196kb)
5 Co-localization/FRET/BRET (.pdf, 137kb)
6 Chromatin Immunoprecipitation (.pdf, 236kb)
7 Electrophoretic Mobility Shift Assay (.pdf, 139kb)
8 Appendix  (.pdf, 113kb)

Index of Guides