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Technical Resources

Guides

Each of our guides is available in .pdf or html format. See our Portable Document Format page if you have difficulty accessing the .pdf file. Certain Guides are also available free from Promega. 
Protocols and Applications Guide NEW!
Bioluminescent Assays for HTS article
Cloning Enzymes
DNA Analysis Notebook 
In Vitro Protein Expression
Nucleic Acid Purification Systems
Protein Interaction
Rabbit Reticulocyte Expression Chapter 
Restriction Enzymes Resource 
RNA Analysis Notebook
Signal Transduction Resource
Subcloning Notebook
Transfection

Bioluminescent Assays for High-Throughput Screening
This article, written by Keith Wood and Frank Fan, originally appeared in ASSAY and Drug Development Technologies, Volume 5, Number 1, 2007.


 

Cloning Enzymes guide cover

Cloning Enzymes
The enzymes highlighted in this guide are those important in nucleic acid cloning procedures.

Note: This Guide is only available online.

DNA Analysis Notebook cover

DNA Analysis Notebook
An introduction to many of the applications related to and products useful in DNA analysis and research. Revised January 2004.

Note: This Guide is only available online.

In Vitro Expression Guide 

In Vitro Protein Expression
A comprehensive guide detailing the technologies and applications associated with in vitro expression. Valuable references and schematics are also included.

Request: Part# BR053

Nucleic Acid Purification Systems Guide

Nucleic Acid Purification Systems
This guide presents many new and innovative products for use in DNA and RNA purification. Revised 9/04.

Request: Part# BR106



Protein Interaction
This guide provides an overview of common techniques used for protein interaction analysis and will assist you in determining which individual technique or combination of techniques is best suited for a particular situation/experiment.

Request: Part# BR188

Rabbit Reticulocyte Expression Systems Chapter
In the broad area of functional genomics, cell-free rabbit reticulocyte lysate (RRL) has been used extensively to elucidate the mechanisms of mammalian translation, cotranslational modifications, post-translational modifications and translocation of proteins. This chapter highlights recent functional proteomics applications that use cell-free mammalian RRL.

Note: This chapter is only available online.

 

Restriction Enzyme Guide

Restriction Enzymes Resource
This interactive guide contains general information relevant to all restriction enzymes, procedures and applications for use and enzyme-specific technical data. Use the guide to find the best choice of enzyme for each situation.

Note: This Guide is an interactive online resource only.



RNA Analysis Notebook
Designed to be an introduction to many of the applications related to and products useful in RNA research.

Request: Part# BR120

Signal Transduction

Signal Transduction Resource
Features overviews of key signaling pathways and the cell signaling reagents Promega offers to help unravel these complex pathways.

Note: This Guide is only available online.

 

Subcloning Notebook
Describes procedures and reagents for use in classic and PCR subcloning, as well as for transforming bacteria and screening for recombinants. Includes a 10-page technical appendix and ordering information.

Note: This Guide is only available online.

Transfection Guide

Transfection
Contains protocols, references and troubleshooting help, and features a cell line table to help you select the Promega transfection reagent that will perform best with commonly-used cell lines.

Note: This Guide is only available online.