Fast
Results

Superior
Sensitivity

Multiplexing
Capability

High Predictive
Values

Sample
Evaluation

Get your results fast without compromising the quality of data gathered.

Promega’s homogeneous cell viability assays get you where you want to go faster with dependable, great results every time.

Compare with your own experience:

Results ready within minutes to hours.

Simple protocols without sample manipulation
or radioactivity.

Sensitive, flexible assays that work dependably time after time.

Consider the benefits, then jump
on the Promega Expressway to GREAT RESULTS.

Get results fast

Our homogeneous add-incubate-read protocols eliminate washing steps or transfer of samples, and are easily automated. Automated protocols are available online.

Reduce incubation times from days to hours or minutes.

Cell Viability Assay	Incubation Time	Detection Method

CellTiter-Glo^® Luminescent Cell Viability Assay	10 minutes	Bioluminescence

CellTiter-Blue™ Cell Viability Assay	1 - 4 hours	Fluorescence

CellTiter 96^® AQueous One Solution Cell Proliferation Assay	1 - 4 hours	Absorbance

CytoTox-ONE™ Homogeneous Membrane Integrity Assay	10 minutes	Fluorescence

View data for Apoptosis Assays

Sensitivity levels that match your needs.

Reduce the minimum number of cells you need to get clear results.

Cell Viability Assays	Parameter Measured	Sensitivity 96-well	Sensitivity 384-well

CellTiter-Glo^® Luminescent Cell Viability Assay	ATP content	50 cells	5 cells

CellTiter-Blue™ Cell Viability Assay	Rezasurin reduction	390 cells	50 cells

CellTiter 96^® AQueous One Solution Cell Proliferation Assay	MTS reduction	800 cells	200 cells

CytoTox-ONE™ Homogeneous Membrane Integrity Assay	LDH release	800 cells	200 cells

View data for Apoptosis Assays

Multiplexing Capability

If you initially measure:		Then you may measure:
Cell Viability		Caspase Activity or General Apoptosis
Initiator Caspase Activity		Executioner Caspase 3/7 Activity
Cytotoxicity or Necrosis		Caspase Activity or General Apoptosis
Reporter Gene Activity		Caspase Activity
Reporter Gene Activity		Cell Viability/Normalization

Contact Technical Services for details

Get high predictive values from Promega's cell viability assays.

Promega's cell viability and apoptosis assays are highly reproducible as measured by Z′ factors. Z′ factor is a statistical value that compares an assay's dynamic range to data variation to assess assay quality. A Z′ factor between 0.5 and 1.0 indicates an excellent assay.

Data for Cell Viability Assays
	CellTiter-Glo^® Assay (ATP)	CellTiter-Blue™ Assay (Resazurin)	CellTiter 96^® AQueous One Solution Assay (MTS)	CytoTox-ONE™ Assay (LDH)

Z′ Values	0.84	0.80	0.71	0.88

%CV	9.1%	3.2%	10%	<5%

# Wells	384	96	96	96

	Reference	Reference	In-house data	Reference

View data for Apoptosis Assays

Caspase Assays Evaluation Request

We'd like to make sure that Promega’s homogeneous caspase assays will deliver the best results for you. Please answer a few short questions about your experience with caspase assays so we can start the evaluation process.
Request a sample kit.
Request Literature about Promega's Caspase Assays.

Superior Sensitivity

^®

Use less enzyme and reduce assay costs.
View Data for: Caspase-Glo 3/7 || Apo-ONE || Caspase-Glo 8 and 9
Use fewer cells and reduce cell culture costs.
View Data for: Caspase-Glo 3/7 || Apo-ONE || Caspase-Glo 8 and 9
Use less compound and save on synthesis costs.
View data for Cell Viability Assays

Apoptosis Assays	Incubation Time	Detection Method

Apo-ONE® Homogeneous Caspase-3/7 Assay	30 minutes -18 hours	Fluorescence

Caspase-Glo™ 3/7 Assay	30 minutes -1 hour	Bioluminescence

Caspase-Glo™ 8 Assay	20 minutes -1 hour	Bioluminescence

Caspase-Glo™ 9 Assay	20 minutes -1 hour	Bioluminescence

Apoptosis Assays

Apo-ONE® Homogeneous Caspase-3/7 Assay	Caspase activity	200 cells	20 cells

Caspase-Glo™ 3/7 Assay	Caspase activity	20 cells	20 cells


If you initially measure…	Then you may measure…

Cell Viability	Caspase Activity or General Apoptosis
CellTiter-Blue™ Cell Viability Assay (fluorescent)	Apo-ONE^® Homogeneous Caspase-3/7 Assay	(fluorescent)
CellTiter-Blue™ Cell Viability Assay (fluorescent, modified procedure)	Caspase-Glo™ 3/7 Assay	(bioluminescent)

Initiator Caspase Activity	Executioner Caspase-3/7 Activity
Caspase-Glo™ 8 Assay (bioluminescent)	Apo-ONE^® Homogeneous Caspase-3/7 Assay	(fluorescent, modified procedure)
Caspase-Glo™ 9 Assay (bioluminescent)	Apo-ONE^® Homogeneous Caspase-3/7 Assay	(fluorescent, modified procedure)

Cytotoxicity or Necrosis	Caspase Activity or General Apoptosis
CytoTox-One Homogeneous Membrane Integrity Assay (fluorescent, modified procedure)	Caspase-Glo™ 3/7 Assay	(bioluminescent)

Reporter Gene Activity	Caspase Activity
EnduRen™ Live Cell Substrate for Renilla luciferase activity (bioluminescent)	Apo-ONE^® Homogeneous Caspase-3/7 Assay	(fluorescent)
	Caspase-Glo™ 3/7 Assay	(bioluminescent)
	Caspase-Glo™ 8 Assay	(bioluminescent)
	Caspase-Glo™ 9 Assay	(bioluminescent)
Contact Technical Services for details


Data for Apoptosis Assays
	Apo-ONE^® Homogeneous Caspase-3/7 Assay (HePG2 cells)	Caspase-Glo™ 3/7 Assay (Caspase-3 Enzyme)	Caspase-Glo™ 8 (Caspase-8 Enzyme) 10µ/ml	Caspase-Glo™ 9 (Caspase-9 Enzyme) 10µ/ml

Z′ Values	0.83	0.92	0.96	0.90

%CV	5%	3.2%	1.4%	3.2%

# Wells	96	96	48	48

	In-House Data	Reference	Reference	Reference

The Caspase-Glo 8 Assay (A) or Caspase-Glo 9 (B) Assay provides superior sensitivity and linearity over a broad range of cell numbers.

General protocol for Promega’s luminescent and fluorescent homogeneous caspase assays.


If you initially measure…	Then you may measure…

Cell Viability	Caspase Activity or General Apoptosis
CellTiter-Blue™ Cell Viability Assay (fluorescent)	Apo-ONE^® Homogeneous Caspase-3/7 Assay	(fluorescent)
CellTiter-Blue™ Cell Viability Assay (fluorescent, modified procedure)	Caspase-Glo™ 3/7 Assay	(bioluminescent)

Initiator Caspase Activity	Executioner Caspase-3/7 Activity
Caspase-Glo™ 8 Assay (bioluminescent)	Apo-ONE^® Homogeneous Caspase-3/7 Assay	(fluorescent, modified procedure)
Caspase-Glo™ 9 Assay (bioluminescent)	Apo-ONE^® Homogeneous Caspase-3/7 Assay	(fluorescent, modified procedure)

Cytotoxicity or Necrosis	Caspase Activity or General Apoptosis
CytoTox-One Homogeneous Membrane Integrity Assay (fluorescent, modified procedure)	Caspase-Glo™ 3/7 Assay	(bioluminescent)

Reporter Gene Activity	Caspase Activity
EnduRen™ Live Cell Substrate for Renilla luciferase activity (bioluminescent)	Apo-ONE^® Homogeneous Caspase-3/7 Assay	(fluorescent)
	Caspase-Glo™ 3/7 Assay	(bioluminescent)
	Caspase-Glo™ 8 Assay	(bioluminescent)
	Caspase-Glo™ 9 Assay	(bioluminescent)
Contact Technical Services for details

The APO-ONE Assay can produce excellent Z′-factor values. Z′-factor value = 0.83 for this assay.

The Caspase-Glo 3/7 Assay can produce excellent Z′-factor values. Z′-factor value = 0.92 for this assay.

The Caspase-Glo 8 Assay can produce excellent Z′-factor values.
Z′-factor Value = 0.957 for this assay.

The Caspase-Glo 9 Assay can produce excellent Z′-factor values.
Z′-factor Value = 0.903 for this assay.

Request Literature about Promega's Caspase Assays


Apo-ONE^® Homogeneous Caspase-3/7 Assay	Caspase-Glo™ 3/7 Assay	Caspase-Glo™ 8-9 Assays	Subscribe to Cell Notes
I want to request all three pieces of literature


If you initially measure…		Then you may measure…
Cell Viability		Caspase Activity or General Apoptosis
Product	Method of Measurement	Product	Method of measurement
CellTiter-Blue™ Cell Viability Assay	fluorescent	Apo-ONE^® Homogeneous Caspase-3/7 Assay	fluorescent

CellTiter-Blue™ Cell Viability Assay	fluorescent, modified procedure	Caspase-Glo™ 3/7 Assay	bioluminescent


If you initially measure…		Then you may measure…
Initiator Caspase Activity		Executioner Caspase-3/7 Activity
Product	Method of Measurement	Product	Method of measurement
Caspase-Glo™ 8 Assay	bioluminescent	Apo-ONE^® Homogeneous Caspase-3/7 Assay	fluorescent, modified procedure

Caspase-Glo™ 9 Assay	bioluminescent	Apo-ONE^® Homogeneous Caspase-3/7 Assay	fluorescent, modified procedure


If you initially measure…		Then you may measure…
Cytotoxicity or Necrosis		Caspase Activity or General Apoptosis
Product	Method of Measurement	Product	Method of measurement
CytoTox-ONE™ Homogeneous Membrane Integrity Assay	fluorescent, modified procedure)	Caspase-Glo™ 3/7 Assay	bioluminescent


If you initially measure…		Then you may measure…
Reporter Gene Activity		Caspase Activity
Product	Method of Measurement	Product	Method of measurement
EnduRen™ Live Cell Substrate for Renilla luciferase activity	bioluminescent	Apo-ONE^® Homogeneous Caspase-3/7 Assay	fluorescent
		Caspase-Glo™ 3/7 Assay	bioluminescent
		Caspase-Glo™ 8 Assay	bioluminescent
		Caspase-Glo™ 9 Assay	bioluminescent


If you initially measure…		Then you may measure…
Reporter Gene Activity		Cell Viability/Normalization
Product	Method of Measurement	Product	Method of measurement
EnduRen™ Live Cell Substrate for Renilla luciferase activity	bioluminescent	CellTiter-Glo^® Luminescent Cell Viability Assay	bioluminescent

Caspase-Glo 3/7 Assay. The Caspase-Glo 3/7 Assay provides superior sensitivity and linearity over 4 orders of magnitude of caspase concentration.

Apo-ONE Assay. Apo-ONE Assay (DEVD-R110) offers superior sensitivity over coumarin-based caspase substrates (DEVD-AFC).

Caspase-Glo 8 and 9 Assays are more sensitive than comparable fluorescent assays, generating large signal windows with broad and linear dynamic ranges.

The Caspase-Glo 3/7 Assay provides superior sensitivity and linearity over a broad range of cell numbers.

Superior sensitivity of the Apo-ONE Assay compared to standard fluorescent caspase assays. Results are obtained in as little as 1 hour.

The Caspase-Glo 8 Assay (A) or Caspase-Glo 9 (B) Assay provides superior sensitivity and linearity over a broad range of cell numbers.