Concordance Study of STR Systems Using the Hitachi FMBIO®-100 Fluorescent Scanner and ABI 310 Capillary Electrophoresis System
N. Mahanti, H. Spillane, L. Helton,
S. Milligan, J. Nye, P. Donald, D. Yet, K. Kuebler, D. Martus, and C. Barna
Michigan State Police DNA Laboratory, 714 South Harrison, East Lansing, MI 48823
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Fluorescently tagged primers for STR analysis have gained wide use in forensic laboratories around the world. Two primary instrumental approaches for detection of the fluorescent products are: 1) Real Time Analysis, 2) Post acrylamide electrophoresis analysis. In this study an ABI 310 capillary electrophoresis system (real time analysis) and a Hitachi FMBIO®-100 fluorescent scanner (post acrylamide electrophoresis analysis) were used to evaluate 150 Michigan Caucasian samples using the PE Applied Biosystems AmpFISTR® Green II and AmpFISTR® Blue systems.
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