Precision Study Using the Hitachi FMBIO®-100 Fluorescent Scanner and Promega 60-400 Base Fluorescent Ladder
N. Mahanti, H. Spillane, L. Helton,
S. Milligan, J. Nye, P. Donald, D. Yet, K. Kuebler, D. Martus, and C. Barna
Michigan State Police DNA Laboratory, 714 South Harrison, East Lansing, MI 48823
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Calibration for STR systems using the FMBIO®-100 fluorescent scanner is routinely accomplished by using a fluorescently tagged calibration ladder. Use of a calibration ladder allows for correction of electrophoretic migration artifacts. In this study the Promega Corporation 60-400 bp ladder labeled with carboxy-x-tetramethylrhodamine (CRX) was used to evaluate the precision of the Hitachi FMBIO®-100 fluorescent scanner and its usefulness in STR analysis.
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