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Short Tandem Repeat (STR) analysis is an important complement to the DNA typing systems already in use for human identification and paternity testing. In the study, the eleven STR loci were used in paternity testing. The determine of STR polymorphism were used the polymerase chain reaction (PCR) and polyacrylamide gel vertical electrophoresis technique by green I staining. The allele bands were identified in the polyacrylamide gel electrophoresis products by sequencing. The discrimination power (D.P) of the eleven STR loci TH01, vWA, F13B, F13A01, LPL, FESFPS, TPOX, CSF1PO, D3S1744, D12S1090, and D18S849 were calculated as 0.86, 0.93, 0.62, 0.68, 0.63, 0.81, 0.73, 0.83, 0.94, 0.97, and 0.89 respectively, in Japanese population. The STR polymorphisms are especially useful genetic markers for identification of individuals and paternity testing.

Upon arrival at the Indianapolis-Marion County Forensic Services Agency, the bloodstains were stored at -20’C until extraction procedures were performed. The DNA was extracted by organic extraction (phenol/chloroform/isoamyl alcohol), followed by ethanol precipitation. The extracted DNA was then quantitated by Quantiblot utilizing the D17Z1 probe. All samples were then subjected to PCR amplification using approximately 2 to 5 ng of DNA.

This study was conducted after a literature search revealed that no DNA data on the Saudi Arabian population has been reported thus far. This study will provide useful scientific data for subsequent forensic and paternity analysis.