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The hypothesis for molecular forensic identification is the invariability of particular DNA sequences in any organ during its lifetime in the same body. However, tumoral changes may cause epigenetic nucleic changes. DNA fingerprinting has proven the variability of some epithelial tumors. However, such testing has never been made on a mesenchymal tumor. In this report, one epithelial tumor and one mesenchymal tumor were selected and analyzed by DNA fingerprinting.

A postoperative liver metastasis of uterus endometrial carcinoma was selected as an example of a malignant epithelial tumor. Samples were taken from the tumor in the liver, the liver infarction and the normal kidney.

Diffuse malignant mesothelioma of the pericaridum was selected as an example of a malignant mesenchymal tumor. Samples were taken from the pericardium, the tumor in the myocardium, the acute centrilobular congestion in the liver and normal kidney.

The DNA samples extracted from these tissues were tested with southern hybridization using probe 33.15 and 33.6. After autoradiography, the density of each band was quantitized and the image analyzed with NIH-Image software.

Probe 33.6 shows both types of tumors displaying about 25-35 polymorphic bands in the 2433-22521 bp region. Analysis of the liver metastasis of uterus endometrial carcinoma shows that the density of some bands increased and that of one band decreased. In the case of malignant mesothelioma, the density of some bands were the reverse of one band and showed a relative increase in the tumoral part. In both tumors, not only the epithelial but also the mesenchymal tumor, some of the DNA bands in the tumoral areas showed greater discoloration or more intense color than those in the normal area, and some bands completely disappeared.